사람뼈 육종세포에서 eugenol에 의해 유발되는 apoptosis의 기작
The mechanism of apoptosis induced by eugenol in human osteosarcoma cells
신상훈, 박재현, 김규천, 박봉수, 길영기, 김철훈,
소속 상세정보
신상훈 ( Shin Sang-Hun ) - 부산대학교 치과대학 구강안면외과학교실
박재현 ( Park Jae-Hyun ) - 부산대학교 치과대학 구강해부학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치과대학 구강해부학교실 및 구강생물공학연구소
박봉수 ( Park Bong-Soo ) - 부산대학교 치과대학 구강해부학교실 및 구강생물공학연구소
길영기 ( Gil Young-Gi ) - 고신대학교 의과대학 해부학교실
김철훈 ( Kim Chul-Hoon ) - 동아의료원 의과대학 구강악안면외과학교실
KMID : 0355620070330010020
Abstract
Eugenol is commonly used in dentistry for the sedation of toothache, pulpitis, and dental hyperalgesia. This study was performed to investigate the apoptotic effect of eugenol to human osteosarcoma (HOS) cells and the potential use of this compound in osteosarcoma cells. Eugenol showed the apoptotic effect in HOS cells in dose- and time-dependent manner. Fragmentation and condensation of DNA were showed by TUNEL assay, Hemacolor stain and Hoechst stain. In the DNA electrophoresis analysis, cells showed DNA degradation characteristic of apoptosis with a ladder pattern of DNA fragments. Apoptosis-related factors were analyzed by western blotting. Cells treated with eugenol showed caspase-3, PARP, lamin A and DFF-45 cleavage. Eugenol treatment induced caspase-3 cleavage and activation. Cleavages of PARP, DFF-45 and lamin A were accompanied with activation of caspase triggered by eugenol in HOS cells. Though this study needs more investigations, these results suggest that eugenol induce apoptosis via caspase dependent pathway in HOS cells and eugenol may constitute a potential antitumor compound against osteosarcoma cells.
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